Scientific and Practical Center for Pathology "HistoGen" performs histological examinations using various staining methods, in the shortest possible time, with maximum diagnostic accuracy.

Histological examination is the study of tissues of humans and other animals using a microscope. Usually, tissues are removed from the body as a result of interventions performed in connection with various diseases (postoperative material). Sometimes, to assess the pathological processes occurring in cells and tissues, a small amount of tissue is also obtained using different needles and technics, without extracting the organ or part of it (biopsy material).

At the first stage of the histological examination, pathologist completely examines the obtained material with the naked eye, assessing the compliance of the samples with the records in the report issued by the physician or surgeon. Then a mandatory gross description is given: size, color, consistency, presence of formations in the sample, etc. Depending on the size of the samples sent for examination, they can be cut into small pieces or examined as a whole (if small fragmented pieces are sent). If parts of tissues remain after cutting out the material, they must be archived and stored in the laboratory as a “wet archive” for a period of 1 year.

Samples taken for microscopic examination go through a series of laboratory processing steps, the scrupulous performance of which directly affects the accuracy of the diagnosis. It is desirable to carry out laboratory processing on automatic equipment, reducing or eliminating the possible influence of the human factor. The various chemicals used in the processing of tissues with their qualitative and quantitative characteristics, also play a key role. The insufficient quantity and poor quality of substances used in laboratories limit the accuracy of morphological evaluation of the sections, makes difficult or impossible subsequent immunohistochemical or molecular studies.

After processing in various chemicals, tissue pieces are embedded in paraffin, "paraffin blocks" are prepared, from which very thin sections 2-4 microns thick are obtained using a microtome. The latter are stained on glass slides by various methods, as directed by the pathologist. Paraffin blocks and slides are stored in the archives of the laboratory for at least 5 years. From one paraffin block, approximately identical sections can be obtained.

At the last stage of histological examination pathologists give conclusions or diagnoses, which serve as the basis for the physician in the management of the patient.